Background and Objective:  The university culture should be changed to an entrepreneurial atmosphere if a university intends to become entrepreneurial.  According to the importance of an entrepreneurial culture and its complications, according to reports from some universities it is inappropriate, this study aimed to evaluate the gap between perceptions and expectations of faculty members of entrepreneurship culture development at the Zahedan University of medical sciences.

Materials and Methods: This is a cross-sectional study. In June2016, a sample of 205 faculty members were stratified randomly selected out of a population of all faculty members of Zahedan University of medical sciences. The Data collection questionnaire entrepreneurship of culture development was with Cronbach's alpha coefficient 0/71 to analyze the data paired samples of T-test which was used by SPSS21.

Results: Average perceptions of the entrepreneurial culture development and its three components (the management factor, human-psychological factor and strategic orientation) were lower than the average expectations of faculty members (P<0/05). Moreove regarding their sex (P>0/05) there was no significant difference between perception and expectations of faculty members of the entrepreneurial culture development .

Conclusion: The results indicate there are significant gaps between the existing and desired situation of culture entrepreneurship development and to reduce these gap, university senior management should pay close attention to the factors affecting culture entrepreneurship development such as management factor, human-psychological factor and strategic orientation.