Background and Objective: Learning based on new technologies has improved educational system deficiencies and brought about major changes in educational system. In order to implement this new method of teaching, this study aimed to determine students' readiness in Zanjan Medical Sciences University.

Materials and Methods:  In a cross-sectional study, e-learning readiness of 120 students was investigated using random stratified sampling. Data collection instruments included demographic and Watkins standard e-learning readiness questionnaire. Readiness was calculated in three low, moderate and good levels. Data was analyzed using descriptive statistics and chi-square test.

Result: By considering all the aspects of e-learning readiness, the results showed the majority of students had a good level of readiness. Readiness of students in 4 aspects of access to technology, motivation, ability to learn through media, online group discussions were moderate and in the aspects of communication skills, major factor of success in e-learning were good. Level of readiness significantly associated with students’ course study (0.001) and educational level (0.006) while this relationship was not significant with the students’ sex, age, and semester.

Conclusion: Despite the fact that the majority of students had a good readiness, the readiness of other elements such as organizations, managers, and teachers also should be considered. Regarding the relationship between demographic factors and readiness, the appropriate intervention to improve the level of readiness, especially among undergraduate students is necessary.